Gibco™

Advanced DMEM

Advanced DMEM (Dulbecco`s Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with자세히 알아보기

Advanced DMEM (Dulbecco`s Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.

This Advanced DMEM is manufactured as follows:

The complete formulation is available.

Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

사양

세포주MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero

분류Animal Origin

농도1 X

형태Liquid

제품 유형DMEM (Dulbecco`s Modified Eagle Medium)

Serum LevelReduced Serum

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

Sterilization MethodSterile-filtered

첨가제 포함High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)

첨가제 없음No Glutamine, No HEPES

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량500 mL

배송 조건Room Temperature

Unit SizeEach