Gibco™

Advanced MEM

Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced자세히 알아보기

Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2.

This Advanced MEM is manufactured as follows:

With	Without
• Glucose	• L-glutamine
• Non Essential Amino Acids	• HEPES
• Sodium pyruvate
• Phenol Red

The complete formulation is available.

Gibco™ Advanced MEM is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Advanced MEM requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

iPaul Price, Brad Stiles, David Staines, Ethel Evege, Femi Fatunmbi, Kate Wagner, Lori Nestler, David Jayme. Advanced Media as Alternatives to Classical Basal Media. FOCUS, 2003. 25.2:3-6.

사양

세포주HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

분류Animal Origin

농도1 X

형태Liquid

제품 유형MEM (Minimum Essential Medium)

Serum LevelReduced Serum

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

Sterilization MethodSterile-filtered

첨가제 포함Low Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)

첨가제 없음No Glutamine, No HEPES

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량500 mL

배송 조건Ambient

Unit SizeEach