Thermo Fisher Scientific TCF12 Polyclonal Antibody
TCF12 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, ChIP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼에 0.02% sodium azide 포함. 냉장 또는 냉동 보관 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 19 amino acid peptide near the amino terminus of human TCF12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152192 |
Product Specific Information
- Suggested positive control: HeLa cell lysate
- Blocking peptide: PEP-1153 compatible with PA5-21039
Target Information
TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG.
It is expressed in various tissues including skeletal muscle, thymus, and B/T cells, and may regulate lineage-specific gene expression through heterodimer formation with other bHLH E-proteins.
TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is essential for T cell differentiation.
Recent studies indicate that TCF12 is also critical for invariant natural killer T cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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