Thermo Fisher Scientific CD14 Monoclonal Antibody (Sa2-8), FITC, eBioscience
마우스 CD14를 인식하는 Sa2-8 단클론 항체로, FITC 형광 표지되어 있습니다. Flow cytometry 분석에 적합하며 488 nm 레이저에서 최적의 형광을 발합니다. 0.5 mg/mL 농도의 액상 형태로 제공되며, 4℃ 암소 보관이 권장됩니다.
- 카탈로그번호
- 11-0141-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD14 Monoclonal Antibody (Sa2-8), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | 1 µg/test | 34 publications |
| Functional Assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Sa2-8 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_464948 |
Additional Available Formats
- APC (Cat. 17-0141-82)
- Biotin (Cat. 13-0141-82)
- PE (Cat. 12-0141-82)
- PE-Cyanine7 (Cat. 25-0141-82)
- PerCP-Cyanine5.5 (Cat. 45-0141-82)
- PerCP-eFluor 710 (Cat. 46-0141-82)
- Super Bright 436 (Cat. 62-0141-82)
- Super Bright 600 (Cat. 63-0141-82)
- Super Bright 645 (Cat. 64-0141-82)
- Custom conjugation available upon request
Product Specific Information
The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53–55 kDa GPI-linked glycoprotein. CD14 acts as a receptor for LPS–LBP complexes and associates with Toll-Like Receptor 4 (TLR4), participating in cellular responses to bacterial LPS. In mice, CD14 is expressed on macrophages and can also be found in serum in a soluble form. The Sa2-8 clone exhibits weak antagonistic activity in NF-κB activation or TNF-α production upon LPS stimulation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of thioglycolate-elicited peritoneal exudate cells. Recommended ≤1 µg/test in 100 µL final volume. Cell count should be empirically determined (10⁵–10⁸ cells/test). Optimize antibody titration for best assay performance.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on monocytes, macrophages, and neutrophils. It functions as a multifunctional receptor for lipopolysaccharide (LPS) in complex with LPS-binding protein (LBP). Soluble CD14 (sCD14) exists in serum and contributes to LPS recognition and neutralization. CD14 also binds apoptotic cells and influences allergic, inflammatory, and infectious processes. Alternative splicing produces multiple transcript variants encoding the same protein. CD14 dysfunction is associated with mycobacterium chelonae infection and croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.