Thermo Fisher Scientific ZIC1/ZIC2/ZIC3 Polyclonal Antibody
ZIC1/ZIC2/ZIC3 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 적합합니다. 인간 및 생쥐 시료 반응성이 있으며, Affinity chromatography로 정제된 액상 항체입니다. PBS/glycerol buffer에 보관하며 -20°C에서 안정적으로 저장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ZIC1 (Accession Q15915), corresponding to amino acid residues G328–R378 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818141 |
Product Specific Information
This antibody detects endogenous levels of total ZIC1/2/3 proteins.
Target Information
Zic1 encodes a zinc finger protein expressed in the developing or mature central nervous system in a highly restricted manner. Zic proteins are expressed in granule cells that make synaptic contact with Purkinje cells and are critical for cerebellar pattern formation.
Expression of Zic genes begins at gastrulation and neurulation, becoming restricted to the dorsal neural ectoderm and dorsal paraxial mesoderm.
Zic1 is mapped to chromosome 9 in mouse, and its 5′ flanking region contains a region-specific enhancer essential for gene regulation.
Zic2 and Zic3 are highly similar to Zic1, with Zic2 essential for brain formation and Zic3 important for right–left axis formation.
The Drosophila odd-paired gene is highly homologous to the Zic gene family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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