Takara McrBC restriction enzyme

McrBC restriction site:

5...(A/G)mC(Nn)(A/G)mC...3
mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine, n=40-2,000

Supplied buffer: McrBC
Reaction temperature: 37°C

More Information

• Source: recombinant proteins expressed in E. coli
• Concentration: 1 unit /µl
• Substrate for unit definition: pBR322
• McrBC specifically cleaves DNA containing methyl cytosine (mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu : A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp. McrBC requires GTP for cleavage.
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Applications

• DNA methylation analysis (qAMP: quantitative analysis of DNA methylation using real-time PCR)
• Enrichment of unmethylated DNA (CHARM: Comprehensive high-throughput arrays for relative methylation)

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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