Takara McrBC restriction enzyme
McrBC는 메틸화된 시토신을 인식하여 절단하는 제한효소로, GTP가 필요하며 40–2,000 bp 거리 내 두 개의 PumC 사이트를 요구합니다. DNA 메틸화 분석 및 비메틸화 DNA 농축에 활용됩니다. 고품질 검증을 거친 재조합 효소입니다.
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McrBC Restriction Enzyme
Restriction Site
5' ...(A/G)mC(Nn)(A/G)mC...3'- mC: 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine
- n: 40–2,000
Reaction Conditions
- Supplied Buffer: McrBC
- Reaction Temperature: 37°C
Specifications
| 항목 | 내용 |
|---|---|
| Source | Recombinant proteins expressed in E. coli |
| Concentration | 1 unit/µl |
| Substrate for unit definition | pBR322 |
| Cleavage specificity | McrBC specifically cleaves DNA containing methyl cytosine (mC: 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu: A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp. McrBC requires GTP for cleavage. |
| Quality testing | Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning |
Applications
- DNA methylation analysis (qAMP: quantitative analysis of DNA methylation using real-time PCR)
- Enrichment of unmethylated DNA (CHARM: Comprehensive high-throughput arrays for relative methylation)
Additional Product Information
Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates in the U.S. and/or other countries.
Additional product, intellectual property, and restricted use information is available at takarabio.com.
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