Takara MboII restriction enzyme
Moraxella bovis 유래 MboII 제한효소로, dam 메틸화에 의해 활성 영향을 받음. 37°C에서 반응하며, lambda DNA를 기준으로 단위 정의. 낮은 cohesive end 라이게이션 효율을 보이며, 블런트 엔드 조건에서 향상 가능. 고품질 QC 수행.
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MboII Restriction Enzyme
Restriction Site
GAAGA(N)8
CTTCT(N)7
Product Specifications
| 항목 | 내용 |
|---|---|
| Supplied Buffer | L |
| Effect of DNA Methylation | Enzyme activity is affected by dam methylase |
| Reaction Temperature | 37°C |
| Source | Moraxella bovis |
| Concentration | 4–12 units/µl |
| Substrate for Unit Definition | Lambda DNA |
| Ligation-Recutting Test | The ligation efficiency of cohesive-ended DNA fragments generated by this enzyme is low. More efficient ligation can be achieved using blunt-end ligation reaction conditions. |
| Quality Testing | Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning |
Additional Product Information
Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components List and Certificates of Analysis are available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates.
Additional product and intellectual property information is available at takarabio.com.
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