Takara MboII restriction enzyme

MboII restriction site:

GAAGA(N)8 |

CTTCT(N)7 |

Supplied buffer: L

Effect of DNA methylation: enzyme activity is affected by dam methylase

Reaction temperature: 37°C

More Information

• Source: Moraxella bovis
• Concentration: 4–12 units/µl
• Substrate for unit definition: lambda DNA
• Ligation-recutting test: The ligation efficiency of cohesive ended DNA fragments generated by this enzyme is low. More efficient ligation can be achieved using blunt end ligation reaction conditions.
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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