Thermo Fisher Scientific CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), eBioscience

Thermo Fisher Scientific CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• Biotin
• Brilliant Violet 421
• Brilliant Violet 711
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and possibly by hematopoietic progenitor cells. It functions as a high-affinity receptor for the hemoglobin-haptoglobin complex, aiding in hemoglobin clearance from blood.

Upon ligand binding, CD163 triggers IL-6 and IL-10 production. Expression is induced by IL-6 and IL-10, while proinflammatory stimuli (LPS, TNF-α) cause shedding of soluble CD163.
This antibody is validated for flow cytometry and Western blot applications. For flow cytometry, ≤0.5 µg per test is recommended (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration is advised for best assay performance.

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein belonging to the scavenger receptor cysteine-rich superfamily. It serves as a receptor for hemoglobin-haptoglobin complexes, protecting tissues from oxidative damage and facilitating iron recycling.

CD163 is expressed on human monocytes and macrophages, particularly during the late phase of inflammation. It is present on all CD14⁺ monocytes and most CD64⁺ monocytes, with higher expression on CD16⁺ subsets. Expression is upregulated by IL-10, IL-6, and dexamethasone, and downregulated by LPS or PMA through shedding of soluble CD163.

Binding of hemoglobin/haptoglobin complexes is calcium- and pH-dependent, with higher affinity for multimeric haptoglobin (HP1F phenotype). CD163 activation induces intracellular signaling cascades involving tyrosine kinase-dependent calcium mobilization, IP₃ production, and secretion of IL-6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.