Thermo Fisher Scientific Caldesmon, HMW (h-Caldesmon) Recombinant Rabbit Monoclonal Antibody (CALD1, 1424R)
Recombinant rabbit monoclonal antibody targeting Caldesmon (h-Caldesmon, CALD1). Validated for WB and IHC applications. Reacts with human and rat samples. High purity, unconjugated form, ideal for smooth muscle research. For research use only.
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Applications
- Western Blot (WB): 1–2 µg/mL
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CALD1, 1424R |
| Immunogen | Recombinant human full-length CALD1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
Target Information
Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Two closely related variants of human caldesmon have been identified, differing in electrophoretic mobility and cellular distribution:
- h-Caldesmon (120–150 kDa): Predominantly expressed in smooth muscle
- l-Caldesmon (70–80 kDa): Found in non-muscle tissue and cells
- Neither variant is detected in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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