Thermo Fisher Scientific CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), Super Bright 436, eBioscience
CD271 (NGF Receptor) 단백질을 인식하는 ME20.4 클론의 Super Bright 436 결합 단일클론 항체. 신경세포 및 줄기세포 연구에 적합하며, Flow Cytometry와 IHC에 사용 가능. 405 nm 레이저에 의해 여기되어 436 nm에서 방출. 연구용으로만 사용.
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Thermo Fisher Scientific CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), Super Bright 436, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ME20.4 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2762623 |
Additional Formats
Product Specific Information
The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa type I transmembrane protein belonging to the low-affinity neurotrophin receptor and TNF receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and certain lymphoid cells including follicular dendritic cells. It binds to NGF, BDNF, NTF3, and NTF4, and contains a death domain that induces apoptosis and regulates cell growth via interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9.
The ME20.4 antibody recognizes CD271 from human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig species.
Applications Reported: Flow cytometry, western blotting, microscopy
Applications Tested: Pre-titrated for flow cytometry using stimulated HEK-293T cells at 5 µL (0.25 µg) per test.
A test is defined as the antibody amount required to stain a cell sample in 100 µL final volume. Cell numbers may range from 10⁵ to 10⁸ cells/test.
Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm. Recommended detection filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Neurotrophic factors regulate the survival, differentiation, and maintenance of neurons in both peripheral and central nervous systems, as well as other neural crest-derived cells. Developing sympathetic neurons require NGF during target competition in vivo. Antibodies specific to NGFR p75 are valuable tools for detecting and characterizing NGFR p75, aiding research into neuronal development, plasticity, and repair mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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