Thermo Fisher Scientific GTF2B Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Target Information

Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment, and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification.

Serial steps for modification include:

1. Activation of ubiquitin, forming a thioester bond between ubiquitin and enzyme E1 (ATP-dependent).
2. Transfer of ubiquitin from E1 to the conjugating enzyme E2.
3. Covalent linkage to the target protein directly by E2 or via E3 ligase enzyme.
4. Deubiquitination enzymes reverse the marking of protein substrates.

Posttranslational tagging by ubiquitin (Ub) is involved in many cellular processes including:

• Cell cycle regulation
• Cell growth and differentiation
• Embryogenesis
• Apoptosis
• Signal transduction
• DNA repair and replication
• Transmembrane transport
• Stress responses
• Immune response
• Nervous system functions

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.