Thermo Fisher Scientific Calnexin Polyclonal Antibody
Calnexin 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, ICC/IF, Flow, IP 등 다양한 응용에 사용 가능. 인간 및 다종 생물 반응성. 고순도 액상 형태로 제공되며 4°C 또는 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Avian, Bovine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide TAPPSSPKVTYKAPVPTGE conjugated to KLH, corresponding to amino acids 50–68 of Human Calnexin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2069041 |
Product Specific Information
Positive control of heat shocked HeLa cell lysate suggested.
Target Information
Calnexin, also known as IP90, p88, and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). It functions as a molecular chaperone involved in proper folding and assembly of polypeptides.
Calnexin associates with MHC class I heavy chains and partial complexes of T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It retains incompletely or improperly folded proteins in the ER.
Unlike soluble ER proteins containing the KDEL sequence, calnexin lacks this motif but has positively charged cytosolic residues ensuring ER retention.
Calnexin structure includes:
- ER luminal domain: 461 amino acids
- Transmembrane segment: 22 amino acids
- Cytoplasmic tail: 89 amino acids
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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