Thermo Fisher Scientific PIK3C2A Polyclonal Antibody
Human PIK3C2A 단백질을 인식하는 Goat Polyclonal Antibody로, IHC(P)에서 5 µg/mL로 검증됨. 합성 펩타이드 면역원 기반으로 제작되었으며, 액상 형태로 0.5 mg/mL 농도 유지. -20°C 보관, 연구용 전용 시약.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (TVKWYQLTAATYL) corresponding to the C-terminus amino acids of PIK3C2A (aa 1674–1686) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10989868 |
Product Specific Information
- Predicted to react with canine and mouse based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
- Preliminary analysis showed no signal in Western Blot.
Target Information
The Class II PI3 kinases, including PIK3C2A, phosphorylate the 3 position of PI or PI4P. Though not as well studied as the Class I PI3 kinases, Class II enzymes are linked to receptor-mediated cellular processes such as insulin signaling, neuronal survival, and growth factor signaling.
PI3-kinases play roles in pathways involved in cell proliferation, oncogenic transformation, cell survival, migration, and intracellular trafficking.
PIK3C2A contains a lipid kinase catalytic domain and a C-terminal C2 domain, characteristic of Class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs mediating membrane translocation and protein-protein interactions.
PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmannin. It can be activated by insulin and may participate in integrin-dependent signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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