Thermo Fisher Scientific Goat anti-Human IgG (H+L) Secondary Antibody, Biotin
Goat anti-Human IgG (H+L) Biotin conjugated secondary antibody로, Western blot, IHC, ICC/IF, ELISA, ISH 등 다양한 실험에 사용 가능. 높은 특이성과 신뢰성으로 인간 IgG 검출에 최적화. 4°C 보관, 1.5 mg/mL 농도.
- 카탈로그번호
- 31770
- 판매단위
- pk
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Thermo Fisher Scientific Goat anti-Human IgG (H+L) Secondary Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:400,000 |
| Immunohistochemistry (IHC) | 1:500–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
| ELISA | 1:200–1:2,000 |
| In Situ Hybridization (ISH) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.8, with 3 mg/mL BSA |
| Contains | 0.08% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_228267 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #31770 has been successfully used in Western blot, IF, ICC, IHC, and ISH applications.
This antibody reacts with the heavy chains of human IgG and the light chains common to most human immunoglobulins, but does not react against non-immunoglobulin serum proteins.
Based on solid-phase enzyme immunoassay, this antibody cross-reacts with horse IgG, human IgM, swine IgG (6%), cat IgG, chicken IgG, dog IgG, dog IgM, guinea pig IgG, mouse IgM, swine IgM (3%), mouse IgG, rabbit IgG, rat IgG, rat IgM (1%), and shows minimal cross-reactivity with immunoglobulins from other species.
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins.
They are useful for detection, sorting, or purification of target molecules.
Secondary antibodies enhance versatility and sensitivity by enabling various detection systems (HRP, AP, fluorescence) and signal amplification.
Typically generated by immunizing host animals with pooled human immunoglobulins, then purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 647
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- AP
- FITC
- HRP
- PE
- Rhodamine
- Texas Red
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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