Thermo Fisher Scientific Goat anti-Human IgM Secondary Antibody, Biotin
Goat 유래 Biotin 결합형 anti-Human IgM 2차 항체로 다양한 면역실험(WB, IHC, ICC, ELISA, ISH)에 사용 가능. 높은 특이성과 낮은 교차반응을 보이며, 안정적인 액상 형태로 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Goat anti-Human IgM Secondary Antibody, Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:400,000 |
| Immunohistochemistry (IHC) | 1:500–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
| ELISA | 1:100–1:500 |
| In Situ Hybridization (ISH) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.8, with 1 mg/mL BSA |
| Contains | 0.08% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient |
| RRID | AB_228278 |
| Target | IgM |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #31778 has been successfully used in Western blot, IF, ICC, IHC, and ISH applications.
This antibody reacts with the heavy chains of human IgM, but does not react against non-immunoglobulin serum proteins.
Based on solid-phase enzyme immunoassay, this antibody cross-reacts with rabbit IgM, swine IgM (25%), rat IgM (12%), guinea pig IgG (6%), mouse IgM (3%), dog IgM (1%), and shows minimal cross-reactivity with immunoglobulins from other species.
Target Information
Anti-Human secondary antibodies are affinity-purified and specifically recognize human immunoglobulins.
They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification.
Typically generated by immunizing host animals with pooled human immunoglobulins, they can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for improved specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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