Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (CT-CD8a), Biotin
Mouse CD8α 단백질을 표적으로 하는 Biotin 결합 단클론 항체로, Flow Cytometry 및 Immunohistochemistry 등 다양한 응용에 적합합니다. Rat IgG2a 아이소타입이며 Protein G로 정제되었습니다. CD8+ T 세포 연구 및 면역학적 분석에 유용합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 1.0 µg/1x10^6 cells | 5 publications |
| Functional Assay (Functional) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CT-CD8a |
| Immunogen | Mouse CD8a |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 4–5 mg/mL BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538985 |
Additional Formats
- Unconjugated (MA5-17594)
- FITC (MA5-17597)
- PE (MA5-17599)
- PE-Cyanine5 (MA5-17601)
- Request custom conjugation
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein belonging to the immunoglobulin family of receptors. It plays a key role in signal transduction, T cell differentiation, and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with T cell receptor (TCR) for MHC class I molecules.
CD8α is essential for MHC-I binding and is also expressed on subsets of T cells, NK cells, monocytes, and dendritic cells as homodimers. Engagement of MHC-I/peptide complexes by antigen-presenting cells recruits Lck kinase, leading to lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are critical for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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