Thermo Fisher Scientific PIST Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 195-454 of human PIST/PIST/GOPC (NP_0010174081) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PIST,uniProtId:Q9HD26-1,ncbiNodeId:9606,antigenRange:195-454,antigenLength:462,antigenImageFileName:PA5-121111_PIST_Q9HD26-1_Rabbit.svg,antigenImageFileNamePDP:PA5-121111_PIST_Q9HD26-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.89 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914683

Product Specific Information

Positive test controls include: HeLa, Mouse kidney, Mouse liver, Mouse brain, Rat brain. The target is usually found in the following locations: Cell junction, Cell projection, Cytoplasm, Golgi apparatus, Golgi apparatus membrane, Peripheral membrane protein, dendrite, postsynaptic cell membrane, postsynaptic density, synapse, trans-Golgi network membrane.

Immunogen sequence: ARLAAKYLDK ELAGRVQQIQ LLGRDMKGPA HDKLWNQLEA EIHLHRHKTV IRACRGRNDL KRPMQAPPGH DQDSLKKSQG VGPIRKVLLL KEDHEGLGIS ITGGKEHGVP ILISEIHPGQ PADRCGGLHV GDAILAVNGV NLRDTKHKEA VTILSQQRGE IEFEVVYVAP EVDSDDENVE YEDESGHRYR LYLDELEGGG NPGASCKDTS GEIKVLQGFN KKAVTDTHEN GDLGTASETP LDDGASKLDD LHTLYHKKSY

Target Information

PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.