Thermo Fisher Scientific PIST Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Positive test controls include HeLa, Mouse kidney, Mouse liver, Mouse brain, and Rat brain.
The target is typically localized in:
Cell junction, Cell projection, Cytoplasm, Golgi apparatus, Golgi membrane, Peripheral membrane protein, dendrite, postsynaptic cell membrane, postsynaptic density, synapse, and trans-Golgi network membrane.

Immunogen sequence:
ARLAAKYLDK ELAGRVQQIQ LLGRDMKGPA HDKLWNQLEA EIHLHRHKTV IRACRGRNDL KRPMQAPPGH DQDSLKKSQG VGPIRKVLLL KEDHEGLGIS ITGGKEHGVP ILISEIHPGQ PADRCGGLHV GDAILAVNGV NLRDTKHKEA VTILSQQRGE IEFEVVYVAP EVDSDDENVE YEDESGHRYR LYLDELEGGG NPGASCKDTS GEIKVLQGFN KKAVTDTHEN GDLGTASETP LDDGASKLDD LHTLYHKKSY

Target Information

PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner.
PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6.
Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.