Thermo Fisher Scientific ATG14 Polyclonal Antibody
ATG14 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, ICC/IF, ELISA에 적합하며 인간, 생쥐, 랫트 반응성. Recombinant ATG14(aa 340-440) 항원으로 제작, 액상 형태로 -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 340–440 of human ATG14 (NP_055739.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.89 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914685 |
Product Specific Information
Positive test controls include: HeLa, HCT116, 293T, SH-SY5Y, Neuro-2a, Mouse testis, Rat testis.
The target is usually found in the following locations: Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Peripheral membrane protein, Preautophagosomal structure membrane, Autophagosome membrane.
Immunogen sequence:
SKQKFTRAVK KLNANILYLC FSQHVNLDQL QPLHTLRNLM YLVSPSSEHL GRSGPFEVRA DLEESMEFVD PGVAGESDES GDERVSDEET DLGTDWENLPS
Target Information
ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene and is located on chromosome 14 in humans. It is also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator).
Autophagy is an evolutionarily conserved process that involves recycling of misfolded cellular proteins and degradation of dysfunctional organelles. It is induced as a cellular response to nutrient stress, including the formation of autophagosomes, fusion with lysosomes, and formation of autophagolysosomes.
The Class III PI3-Kinase Vps34 and its interacting partner Beclin 1 form a complex that specifically includes ATG14 (type 1) or UVRAG (type 2). Atg14 guides the type 1 complex to the preautophagosomal structure (PAS) and is critical for initiating autophagosome formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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