Thermo Fisher Scientific PTGER3 Polyclonal Antibody, Biotin
PTGER3 단백질을 인식하는 Biotin-conjugated Rabbit Polyclonal 항체. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. Human 및 Mouse 시료 반응성. Affinity chromatography로 정제된 고순도 제품. -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 300–350 of human Prostaglandin E receptor 3 (subtype EP3) protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD172a, also known as signal-regulatory protein alpha, is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells, macrophages, mast cells, and hematopoietic stem cells.
It acts as a substrate for several activated tyrosine kinases (EGFR, PDGFR, src, insulin receptor) and participates in the negative regulation of receptor tyrosine kinase-coupled signaling pathways.
Ligand binding of CD172a to integrin-associated protein CD47 leads to phosphorylation of ITIMs within the cytoplasmic region, recruiting tyrosine phosphatases SHP-1 and SHP-2.
This interaction regulates cellular functions such as inhibition of macrophage phagocytosis and bidirectional activation of T cells and dendritic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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