Thermo Fisher Scientific CDC42BPB Monoclonal Antibody (2F4)
CDC42BPB 단백질을 인식하는 Mouse 모노클로날 항체(클론 2F4)로, Western Blot, IHC, ICC, ELISA 등 다양한 분석에 적합합니다. 인체 및 랫트 반응성을 가지며, 액상 형태로 제공되며 PBS buffer에 보존됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CDC42BPB Monoclonal Antibody (2F4)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F4 |
| Immunogen | CDC42BPB (AAD37506, 1580–1679 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
SKMISNPTNF NHVAHMGPGD GMQVLMDLPL SAVPPSQEER PGPAPTNLAR QPPSRNKPYI SWPSSGGSEP SVTVPLRSMS DPDQDFDKEP DSDSTKHSTP
Target Information
CDC42BPB is a serine/threonine kinase involved in actin cytoskeleton assembly and organization.
Myotonic dystrophy kinase-related Cdc42-binding (DMPK-like) kinases—MRCK-alpha and MRCK-beta—contain a cysteine-rich motif and a pleckstrin homology domain.
MRCKs phosphorylate nonmuscle myosin light chain, influencing actin-myosin contractility.
MRCK-alpha can phosphorylate and activate LIM kinases downstream of Cdc42, leading to ADF/Cofilin inactivation and actin cytoskeletal reorganization.
Additionally, MRCK-alpha modulates neurite outgrowth promoted by Cdc42 and Rac.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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