Thermo Fisher Scientific HNF1-alpha Polyclonal Antibody
HNF1-alpha 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 적합. 고순도 Liquid 형태로 1 mg/mL 농도 제공. FFPE 조직의 IHC에 citrate buffer(pH 6.0) 전처리 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 550 to 600 of human HNF1 Homeobox A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region and may play a role in the phenotype of WHS or Pitt-Rogers-Danks syndrome. The encoded protein can react with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for many cancer patients. It is also a member of the NELF (negative elongation factor) protein complex involved in RNA polymerase II transcription elongation regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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