Thermo Fisher Scientific Neuraminidase (Neu2) Polyclonal Antibody
Human Neu2 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, IHC, ELISA, IP에 사용 가능. Affinity chromatography로 정제된 액상 항체이며, 0.9 mg/mL 농도. -20°C에서 보관하며, 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum immunized with a synthetic peptide corresponding to an internal portion near aa 100–125 of Human Neu2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening.
Aliquot contents and freeze at -20°C or below for extended storage.
Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
This antibody reacts with human Neu2. Based on sequence, it is expected to react with neuraminidase from other species, although specific reactivity has not been confirmed. Cross-reactivity against Neu1 has not yet been established. Neuraminidases are highly conserved in mammals; therefore, cross-reactivity is expected with mouse and rat Neu2.
Target Information
This gene belongs to a family of glycohydrolytic enzymes that remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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