Thermo Fisher Scientific Kir2.1 (KCNJ2) Polyclonal Antibody
Rabbit polyclonal antibody targeting human Kir2.1 (KCNJ2). Validated for WB, IHC(F), ICC/IF, and IP. Lyophilized form for stable storage at -20°C. Supplied unconjugated, concentration 0.8 mg/mL. For research use only.
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Thermo Fisher Scientific Kir2.1 (KCNJ2) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC(F)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | Assay-Dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide (C)NGVPESTSTDTPPDIDLHN, corresponding to amino acid residues 392–410 of human Kir2.1 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2736035 |
Product Specific Information
Product is shipped at room temperature as a lyophilized powder and should be stored at -20°C upon receipt.
Reconstitution: add 50 µL of deionized water.
Target Information
The KIR2 subunit family includes 2.1, 2.2, 2.3, and 2.4.
Unlike G protein-coupled KIR3 subunits, KIR2.1 requires both phosphorylation by PKA and ATP hydrolysis for functional activity.
KIR2.1 is expressed in the superior and inferior colliculi and the pontine region of the brain, moderating synaptic transmission like other potassium channels.
In the placenta, KIR2.1 is expressed throughout gestation in cytotrophoblast cells.
In the kidney, KIR2.1 colocalizes with KIR5.1 in the proximal tubule.
KIR2.1, 2.2, and 2.3 associate with the membrane-associated guanylate kinase synapse-associated protein 97 (SAP97) in the cerebellum and heart.
Phosphorylation of KIR2.2 by protein kinase A inhibits its association with SAP97.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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