
Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, AP
Donkey 유래 Mouse IgG(H+L) 결합형 2차항체로, AP 효소 결합 및 고도의 교차흡착 처리로 비특이적 결합 최소화. Western blot, IHC, ELISA 등 다양한 면역실험에 적합. 높은 순도와 안정적 신호 증폭 제공.
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Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, AP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534694 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 680
- Alexa Fluor Plus 800
- Biotin
- FITC
- HRP
- TRITC
- Request custom conjugation available
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies provide versatility and enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically produced by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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