Thermo Fisher Scientific AFP Monoclonal Antibody (OTI1B3), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract, and liver during fetal life.
In adults, AFP expression is often associated with hepatoma or teratoma, though hereditary persistence can occur without pathology.
AFP functions as the fetal counterpart of serum albumin, with both genes located tandemly on chromosome 4.
It exists in monomeric, dimeric, and trimeric forms, and can bind copper, nickel ions, fatty acids, and bilirubin.
AFP levels in amniotic fluid are used to screen for spina bifida and anencephaly, and elevated serum AFP is associated with hepatocellular carcinoma, teratoblastoma, colorectal, pancreatic cancer, and germ cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.