Thermo Fisher Scientific FOXP3 Monoclonal Antibody (OTI8H4), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

FOXP3 (Forkhead box protein 3) is a transcriptional regulator belonging to the forkhead/winged-helix family, highly conserved across mammals and critical for immune homeostasis.
It is expressed at high levels in CD25⁺CD4⁺ regulatory T cells, at low levels in CD4⁺/CD25⁻ cells, and absent in CD4⁻/CD8⁺ T cells.
FOXP3 functions as a master regulatory gene and a specific marker for regulatory T cells.
Mutations in FOXP3 cause the scurfy phenotype in mice and are associated with IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) in humans.
Expression of FOXP3 in human breast and colon cancer cells is regulated by p53 in response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.