Thermo Fisher Scientific Vinculin Polyclonal Antibody
Thermo Fisher Scientific의 Vinculin Polyclonal Antibody는 인간 및 생쥐 시료에 반응하며 Western blot, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 고품질 액상 항체로, 세포골격 단백질 연구에 최적화되어 있습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 5–15 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1016 and 1066 of Human vinculin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Predicted to react with Rat, Chicken, and Pig (based on 100% sequence identity)
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. Vinculin interacts with talin, integrin β subunit, and α-actinin, forming a key linkage between the extracellular matrix and the cytoplasmic microfilament system. Mutations in the vinculin gene can lead to cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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