Thermo Fisher Scientific Vinculin Polyclonal Antibody
Western blot 및 Immunoprecipitation에 사용 가능한 Rabbit Polyclonal 항체. Human과 Mouse에 반응하며, Rat, Chicken, Pig에서도 예측 반응. 항원 친화 크로마토그래피로 정제된 액상 항체로 4°C에서 보관. 세포 부착 관련 단백질 Vinculin 검출에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 750 and 800 of Human vinculin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken, and Pig
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell–cell and cell–extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions is involved in the transmembrane assembly of adhesion plaques: the beta subunit of integrin binds to talin, talin binds to vinculin, which interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the linkage between the extracellular matrix and the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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