Thermo Fisher Scientific Frizzled 5/8 Polyclonal Antibody
Frizzled 5/8 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot, IHC, ICC, Flow cytometry, ELISA 등에 사용 가능. Human, Mouse, Rat 반응성. Protein A 정제, 액상 형태로 -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Frizzled 5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Members of the frizzled gene family encode 7-transmembrane domain proteins that serve as receptors for Wnt signaling proteins. The Frizzled 5 protein is believed to be the receptor for the Wnt5A ligand. It has been reported to be expressed in fetal kidney, fetal and adult liver, fetal lung, and adult pancreas. ESTs have been isolated from bone, liver/spleen, placenta, and prostate libraries. Frizzled 5 was cloned from a retina cDNA library.
Frizzled 5 functions as a receptor for Wnt proteins and is part of the Wnt-Fzd-LRP5-LRP6 complex that triggers β-catenin signaling through aggregation of receptor-ligand complexes. This canonical pathway activates disheveled proteins, inhibits GSK-3 kinase, promotes nuclear accumulation of β-catenin, and activates Wnt target genes. Some family members also signal through PKC and calcium fluxes, possibly integrating with the canonical pathway. Both pathways involve G-protein interactions and may contribute to polarity information transmission during tissue morphogenesis and differentiation. Frizzled 5 also acts as a coreceptor with RYK for Wnt proteins such as WNT1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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