Thermo Fisher Scientific TNFRSF5 Polyclonal Antibody
TNFRSF5(CD40)에 특이적인 Rabbit Polyclonal Antibody로 다양한 응용(WB, IHC, ICC, Flow, ELISA)에 사용 가능. Human, Mouse, Rabbit, Rat 반응성. Protein A로 정제된 액상 항체이며 -20°C 보관. 면역 및 염증 반응 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human TNFRSF5 (amino acids 201–277) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD40 antigen is a single-chain glycoprotein belonging to the tumor necrosis factor/nerve growth factor superfamily. It shows significant homology to CD30 and is expressed on all B cells except plasma cells, as well as on some epithelial cells, carcinomas, and lymphoid dendritic cells.
CD40 plays an essential role in immune and inflammatory responses, including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
Transcription factor AKNA regulates CD40 expression, and adaptor protein TNFR2 mediates signal transduction.
The CD40–CD40L interaction is necessary for amyloid-beta-induced microglial activation, implicated in Alzheimer’s disease pathogenesis.
Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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