Thermo Fisher Scientific p70 S6 Kinase Polyclonal Antibody
Human p70 S6 Kinase에 특이적인 Rabbit Polyclonal Antibody로 Western blot, ICC/IF, IP에 사용 가능. 단백질 합성 및 세포 증식 연구에 적합하며, 보존제가 포함되지 않아 필요 시 sodium azide 첨가 권장. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:300–1:10,000 |
| Immunoprecipitation (IP) | 1–4 µL/mg of lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human PS6K/RPS6KB1 Protein (Met1–Leu525) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2788237 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human PS6K/RPS6KB1.
Target Information
RPS6KB1 (p70S6K) is a serine/threonine kinase with two non-identical kinase catalytic domains that phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm and is essential in growth factor-regulated cell proliferation, cell motility pathways (such as metastasis), immune response, and tissue repair.
It acts downstream of phosphoinositide (PI) 3-kinase, with its main physiological target being the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to increased protein synthesis and cell proliferation. Amplification and overexpression are observed in some breast cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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