Thermo Fisher Scientific RUNX2 Monoclonal Antibody (3F5)
RUNX2 단백질을 인식하는 mouse monoclonal antibody로, 인간 시료에 반응합니다. Western blot, IHC, ICC/IF, ELISA에 사용 가능하며, 골 형성 및 RUNX2 관련 연구에 적합합니다. 액상 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3F5 |
| Immunogen | RUNX2 (NP_004339, 251–350 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA
Target Information
RUNX2 is a nuclear protein and member of the RUNX family of transcription factors containing a Runt DNA-binding domain. It is essential for both membranous and endochondral bone formation. RUNX2 regulates osteoblastic differentiation and skeletal morphogenesis, functioning as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. It binds DNA as a monomer or as part of a heterodimeric complex. RUNX2 plays a key role in increasing TGFBR1 expression by osteoblasts and cooperates with DLX5 or related factors to activate osteoblast-specific gene expression. Mutations in RUNX2 can cause cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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