
Thermo Fisher Scientific P4HA1 Polyclonal Antibody
P4HA1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(Paraffin)에 적합합니다. Human 시료에 반응하며 Mouse, Rat 등에서도 예측 반응성이 높습니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼에 BSA와 글리세롤이 포함되어 안정성이 우수합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | – |
| Immunohistochemistry (IHC) | – | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 318–534 of Human P4HA1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.18 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2548720 |
Product Specific Information
- Recommended positive control: PC-3
- Predicted reactivity: Mouse (97%), Rat (95%), Pig (99%), Chicken (96%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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