Thermo Fisher Scientific ABI1 Polyclonal Antibody
Rabbit polyclonal antibody against human ABI1 protein. Validated for WB, IHC, and IP applications. Recommended dilutions: WB 1:2,000–1:10,000; IHC 1:100–1:500. Suitable for mouse and rat cross-reactivity. Supplied as liquid, 0.20 mg/mL, store at 4°C.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | |
| Immunohistochemistry (IHC) | 1:100–1:500 | |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 | Epitope retrieval with citrate buffer pH 6.0 recommended for FFPE tissue |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 325 and 375 of human abl-interactor 1 (ABI1, UniProt ID: Q8IZP0-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on sequence identity, predicted to react with Mouse and Rat
Target Information
This gene encodes a member of the DEAD box protein family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), which are putative RNA helicases. These proteins are implicated in processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Some members are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors and represses their transcriptional activity. Alternative splice variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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