Thermo Fisher Scientific Phospho-MEK1 (Thr286) Polyclonal Antibody
Phospho-MEK1 (Thr286) 특이적 폴리클로날 항체로, Human, Mouse, Rat에 반응합니다. WB, IHC, ICC, ELISA 등 다양한 응용에 적합하며, Protein A로 정제된 고순도 항체입니다. 세포 신호전달 연구용으로 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Thr286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, regulating cell growth and differentiation. It is activated by various growth factors, cytokines, membrane depolarization, and calcium influx. MEK1 activation occurs via phosphorylation at serine 218 and 222 residues by Raf1. It plays a role in stress response, apoptosis, and cytokine-induced proliferation. Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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