Thermo Fisher Scientific TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody (1E8-G6)
TGF-alpha 단백질을 인식하는 마우스 단클론 항체로, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능. Human, Rabbit, Zebrafish에 반응하며, Protein A/G 정제 및 PBS 버퍼에 보관. 연구용으로 세포 증식 및 성장 인자 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Rabbit, Zebrafish |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E8-G6 |
| Immunogen | A 10-amino acid synthetic peptide (aa34–43, PPVAAAVVSH) from human TGF-alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independent transformation in cultured cells. Human, murine, and rat TGF-alpha are cross-species reactive. TGF-alpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing a reversible transformed phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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