
Thermo Fisher Scientific alpha Amylase Polyclonal Antibody, Biotin
Bacillus amyloliquefaciens 유래 α-Amylase를 인식하는 Biotin 결합 토끼 다클론 항체. Western blot, ELISA, IP에 적합. 고순도 이온교환 크로마토그래피 정제. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | α-Amylase (Bacillus amyloliquefaciens) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-Alpha Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the specified buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, as well as purified and partially purified α-Amylase (B. amyloliquefaciens).
Cross-reactivity against α-Amylase from other sources may occur but has not been specifically determined.
Target Information
Amylases are secreted proteins that hydrolyze 1,4-α-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen.
The human genome contains several amylase genes expressed at high levels in either the salivary gland or pancreas. It is an amylase isoenzyme produced by the salivary gland.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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