Thermo Fisher Scientific COX4I2 Monoclonal Antibody (1F2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

3 µg/mL

-

ELISA (ELISA)

0.03 ng/mL

-

Immunoprecipitation (IP)

1-5 µg/mL

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1F2

Immunogen

COX4I2 (NP_115998, 21 a.a. approximately 104 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:COX4I2,uniProtId:Q96KJ9-1,ncbiNodeId:9606,antigenRange:21-104,antigenLength:171,antigenImageFileName:H00084701-M01_COX4I2_Q96KJ9-1_House_mouse.svg,antigenImageFileNamePDP:H00084701-M01_COX4I2_Q96KJ9-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MHSSEGTTRG GGKMSPYTNC YAQRYYPMPE EPFCTELNAE EQALKEKEKG SWTQLTHAEK VALYRLQFNE TFAEMNRRSN EWKT

Target Information

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunbit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [Uniprot]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.