Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17), Alexa Fluor Plus 555
DYKDDDDK(FLAG) 태그 단백질 검출용 Alexa Fluor Plus 555 결합 재조합 토끼 단클론 항체. Western blot, ICC/IF, Flow cytometry에 적합. 높은 특이성과 민감도, 일관된 로트 간 재현성 제공. 4°C 보관, 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:400 |
| Flow Cytometry (Flow) | 0.1 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8H8L17 |
| Immunogen | Synthetic peptide (DYKDDDDK) |
| Conjugate | Alexa Fluor™ Plus 555 |
| Excitation / Emission Max | 558 / 572 nm |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2942245 |
Additional Formats
- Unconjugated
- Alexa Fluor Plus 488
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Biotin
- HRP
- Custom conjugation available upon request
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include superior specificity and sensitivity, lot-to-lot consistency, and broader immunoreactivity due to the rabbit immune repertoire.
Alexa Fluor™ Plus antibodies provide brighter signals and improved signal-to-noise ratios compared to previous Alexa Fluor conjugates, enabling superior imaging performance. These antibodies use proprietary dye chemistry for enhanced fluorescence.
Usage Note:
Centrifuge the conjugate solution briefly before use and use only the supernatant to minimize aggregates and nonspecific staining. Optimal antibody dilutions should be determined empirically for each application.
Applications Tested:
Validated for immunoblotting, immunocytochemistry, and flow cytometry of DYKDDDDK-tagged proteins.
Recommended to titrate for optimal assay performance.
Optical Properties:
Excitation: 553 nm
Emission: 568 nm
Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The DYKDDDDK tag (FLAG tag) is an eight–amino acid peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) widely used for protein purification, detection, and localization. It can be fused to either terminus of a protein to facilitate identification and isolation using anti-FLAG antibodies.
The FLAG tag is hydrophilic, minimizing interference with protein structure and activity.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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