Thermo Fisher Scientific Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody
p38 MAPK의 Thr180/Tyr182 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, 스트레스 반응 및 염증 관련 신호전달 연구에 적합합니다. WB, IHC, ICC/IF 등에 사용 가능하며, 고순도 친화 크로마토그래피로 정제되었습니다. -20°C에서 보관하십시오.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 12 publications |
| Immunohistochemistry (IHC) | 1:250 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:250 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to a region surrounding the phosphorylated Thr180/Tyr182 residues of p38 MAP kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533281 |
Product Specific Information
This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primates, and zebrafish based on 100% sequence homology.
Target Information
MAPK14 (p38 alpha) belongs to the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates various cellular responses to stress, inflammation, and other stimuli. It is inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of Thr-Gly-Tyr motifs. Four isoforms of p38 MAPK differ in tissue expression and affinity for activators and effectors. Upon exposure to tumor necrosis factor, interleukin-1, heat shock, or other stimuli, MAPK kinase-3/6 phosphorylates Thr180 and Tyr182 residues of p38 MAPK, leading to activation of transcription factors such as ATF-2, CHOP-1, and MEF-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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