Thermo Fisher Scientific Bromodeoxyuridine (BrdU) (Proliferation Marker) Monoclonal Antibody (BU20a)
세포 증식 마커 BrdU를 검출하기 위한 Thermo Fisher Scientific의 단클론 항체입니다. 다양한 면역염색 및 유세포분석에 사용 가능하며, 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BU20a |
| Immunogen | Bromodeoxyuridine (BrdU) conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside and an analogue of thymidine. BrdU is commonly used for detecting proliferating cells in living tissues, as it can be incorporated into newly synthesized DNA during the S phase of the cell cycle, substituting for thymidine during replication. Antibodies specific for BrdU detect the incorporated BrdU, indicating cells actively replicating their DNA. Binding of the antibody requires DNA denaturation, typically achieved by acid or heat treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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