Thermo Fisher Scientific Cytokeratin, Basic (Type II or HMW) Monoclonal Antibody (34BE12)
인간, 마우스, 랫트 반응성을 가진 단클론 항체로 다양한 세포 및 조직 염색에 사용 가능. Western blot, IHC, ICC/IF, Flow cytometry 등 다중 응용에 적합. 단백질 A/G 정제, PBS 버퍼 저장, 보존제 없음. 연구용으로만 사용.
- 판매단위
- pk
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Thermo Fisher Scientific Cytokeratin, Basic (Type II or HMW) Monoclonal Antibody (34BE12)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 34BE12 |
| Immunogen | Solubilized keratin extract from human stratum corneum |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cytokeratin pan belongs to a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40–68 kDa and have isoelectric points between pH 4.9–7.8. Human cytokeratins are numbered from 1 to 20.
Different epithelia express distinct cytokeratin profiles that correlate with tissue type and differentiation stage. These expression patterns are valuable in distinguishing epithelial malignancies. Cytokeratin antibodies assist in tumor diagnosis using immunohistochemistry and are useful in cytopathology and flow cytometry assays. Expression varies with cell type, growth environment, and disease state, making keratins important markers in cancer research and diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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