Thermo Fisher Scientific EphB4 Monoclonal Antibody (3D7F8)
EphB4 단백질을 표적으로 하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 3D7F8). Western blot, IHC, ICC/IF, ELISA 등 다양한 연구용 응용에 적합. Human 및 Mouse 반응성 확인. Protein A 정제, PBS buffer에 0.1% sodium azide 포함.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20 | - |
| Immunocytochemistry (ICC/IF) | 3–5 µg/mL | View 1 publication |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D7F8 |
| Immunogen | Recombinant protein corresponding to the C-terminal sequence of the human EphB4 receptor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533201 |
Product Specific Information
- Clone 37-1800 has been successfully used in Western blot, immunohistochemical, immunofluorescence, and ELISA assays.
- Clone 35-2900 detects a ~125 kDa band representing EphB4 and a strong band at ~90 kDa, likely another ephrin family protein.
- Clone 37-1800 is recommended for Western blot applications.
Target Information
EPHB4 is a receptor tyrosine kinase belonging to the ephrin receptor family. Eph receptors are key regulators in processes such as:
- Nervous system development
- Angiogenesis
- Neural synapse formation and maturation
Ephrins are divided into two classes:
- Ephrin-A (EFNA): Anchored via glycosylphosphatidylinositol linkage
- Ephrin-B (EFNB): Transmembrane proteins
Eph receptors are similarly grouped based on extracellular domain sequence and ligand affinity. EphB4 specifically binds ephrin-B2 and plays a crucial role in vascular development.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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