Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), PE, eBioscience
인간 CD4 항원 인식용 OKT4 단클론 항체로, PE 형광 표지되어 유세포분석(Flow Cytometry)에 최적화됨. HIV 수용체 연구 및 T-helper 세포 분석에 적합. 고순도 친화성 크로마토그래피 정제, 4°C 암소 보관.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), PE, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.06 µg)/test
- Publications: View 32 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT4 (OKT-4) |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2016675 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant Violet 650
- Brilliant Violet 786
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Yellow 660
- PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by thymocytes, mature T-helper cells, and at low levels on monocytes. CD4 acts as a receptor for HIV. OKT4 recognizes a distinct epitope from RPA-T4 and does not cross-block.
This antibody is validated for flow cytometric analysis and can be used at 5 µL (0.06 µg) per test.
Each test corresponds to staining a cell sample in 100 µL final volume (typically 10⁵–10⁸ cells/test).
Excitation: 488–561 nm; Emission: 578 nm; Compatible lasers: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD4 is involved in recognition of MHC class II molecules and serves as a co-receptor for HIV. It is mainly expressed on T-helper cells and also found on monocytes, macrophages, and dendritic cells.
Tissue expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of brain and gut.
CD4 functions in T-cell activation via association with the TCR complex and Lck kinase, and may mediate neuronal damage in infectious or immune-related CNS diseases.
Multiple alternatively spliced transcripts are known (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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