Thermo Fisher Scientific Phospho-Bid (Ser65) Polyclonal Antibody
Phospho-Bid (Ser65) 폴리클로날 항체로 Rat 시료에 반응하며, Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. KLH 결합 합성 인산화 펩타이드로부터 제작되었으며, 고순도 Protein A 정제 및 안정적 PBS/glycerol 저장 buffer를 사용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from rat Bid around the phosphorylation site of Ser65 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. It is a member of the BCL-2 family of cell death regulators and mediates mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria, triggering cytochrome c release. BID usually exists in an inactive form in the cytosol and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. The cleaved C-terminal fragment (p15) is sufficient to trigger cytochrome c release, resulting in apoptosis. BID acts as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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