Thermo Fisher Scientific FH Monoclonal Antibody (5C12)
Thermo Fisher Scientific FH Monoclonal Antibody (5C12)는 인간 FH 단백질을 인식하는 Mouse IgG2a 형 단일클론 항체입니다. Western blot, ICC/IF, ELISA에 사용 가능하며, GST 태그가 포함된 full-length FH 단백질을 면역원으로 제작되었습니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C12 |
| Immunogen | FH (AAH03108, 33 a.a. approximately 510 a.a) full-length recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
VPSFWPPNAA RMASQNSFRI EYDTFGELKV PNDKYYGAQT VRSTMNFKIG GVTERMPTPV IKAFGILKRA AAEVNQDYGL DPKIANAIMK AADEVAEGKL NDHFPLVVWQ TGSGTQTNMN VNEVISNRAI EMLGGELGSK IPVHPNDHVN KSQSSNDTFP TAMHIAAAIE VHEVLLPGLQ KLHDALDAKS KEFAQIIKIG RTHTQDAVPL TLGQEFSGYV QQVKYAMTRI KAAMPRIYEL AAGGTAVGTG LNTRIGFAEK VAAKVAALTG LPFVTAPNKF EALAAHDALV ELSGAMNTTA CSLMKIANDI RFLGSGPRSG LGELILPENE PGSSIMPGKV NPTQCEAMTM VAAQVMGNHV AVTVGGSNGH FELNVFKPMM IKNVLHSARL LGDASVSFTE NCVVGIQANT ERINKLMNES LMLVTALNPH IGYDKAAKIA KTAHKNGSTL KETAIELGYL TAEQFDEWVK PKDMLGPK
Target Information
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid cycle (Krebs cycle) and catalyzes the formation of L-malate from fumarate.
It exists in both cytosolic and N-terminal extended forms, differing only in the translation start site used.
The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm.
It functions as a homotetramer and is similar to thermostable class II fumarases.
Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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