Thermo Fisher Scientific DDDDK Tag Polyclonal Antibody
Rabbit polyclonal antibody recognizing DDDDK (FLAG) tag for protein detection and purification. Validated for WB and ELISA applications. Supplied as liquid, 1 mg/mL, purified by Protein A. For research use only.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:2,000
ELISA
- Tested Dilution: 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAP of Flag Tag peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used as a protein tag to facilitate protein purification, detection, and localization.
The tag consists of eight amino acids: Asp–Tyr–Lys–Asp–Asp–Asp–Asp–Lys.
It is often fused to the N- or C-terminus of a protein of interest, allowing easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely used in molecular biology and biochemistry research for applications such as:
- Protein–protein interaction studies
- Protein expression analysis
- Protein localization studies
The FLAG tag structure is optimized for compatibility with attached proteins, being more hydrophilic than other epitope tags and less likely to denature or inactivate proteins.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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