Thermo Fisher Scientific DDDDK Tag Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:2,000

ELISA

• Tested Dilution: 1:500–1:1,000

Product Specifications

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used as a protein tag to facilitate protein purification, detection, and localization.
The tag consists of eight amino acids: Asp–Tyr–Lys–Asp–Asp–Asp–Asp–Lys.
It is often fused to the N- or C-terminus of a protein of interest, allowing easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely used in molecular biology and biochemistry research for applications such as:

• Protein–protein interaction studies
• Protein expression analysis
• Protein localization studies

The FLAG tag structure is optimized for compatibility with attached proteins, being more hydrophilic than other epitope tags and less likely to denature or inactivate proteins.

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.