Thermo Fisher Scientific CYLD Polyclonal Antibody
CYLD 단백질을 표적하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat에 반응합니다. WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합하며 Protein A로 정제된 고순도 항체입니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific CYLD Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 2 µg/Test |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human cylindromatosis 1, amino acids 501–600 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CYLD is a 956 amino acid cytoplasmic deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family. It contains three CAP-GLY domains, a proline-rich region, a SH3 binding domain, and a catalytic domain homologous to UCH proteins. CYLD acts as a tumor suppressor protein influencing the JNK signaling pathway and negatively regulates TRAF2 and NF-kappa-B signaling. It also plays a receptor-dependent role in regulating the I-kappa-B kinase pathway and exhibits deubiquitinating activity toward non-Lys-48-linked polyubiquitin chains. Mutations in CYLD are associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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