Thermo Fisher Scientific HCN2 Polyclonal Antibody
HCN2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF에 사용 가능. 합성 펩타이드 면역원으로 제작되어 높은 특이성과 재현성을 제공. 뇌와 심장 조직 연구에 적합하며, 장기 보관 시 -20°C에서 안정적으로 보존 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:300–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from mouse HCN2 conjugated to blue carrier protein was used as the antigen. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2248014 |
Product Specific Information
- Glycerol (1:1) may be added for additional stability.
- Reconstitute in 100 µL of sterile water.
- The peptide is homologous in rat.
Target Information
Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.
HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides.
HCN1 and HCN2 play an important role in motor learning and neuronal integration by cerebellar Purkinje cells, as well as shaping autonomous activity of single neurons and the periodicity of network oscillations.
HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus.
HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body.
These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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