Thermo Fisher Scientific CD157 Monoclonal Antibody (eBioSY11B5 (SY11B5)), NovaFluor Yellow 610, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 590
• PE
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The eBioSY11B5 monoclonal antibody recognizes human CD157 (Mo5, BST-1). CD157 is a 42–45 kDa, GPI-anchored protein structurally and functionally related to CD38. It was first identified on monocytes and macrophages and later found to be identical to BST-1, expressed on bone marrow stromal cells. CD157 functions as both an ectoenzyme and receptor, influencing NAD⁺ metabolism and intracellular Ca²⁺ regulation. Upon ligand binding, it can trigger phosphorylation of cytoplasmic proteins such as focal adhesion kinase (FAK).

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per 100 µL sample (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
• Add 5 µL of CellBlox Plus per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL cell sample.

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures engineered for optimized fluorescence with minimal spillover and spread.

Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

Target Information

Bone marrow stromal cell antigen 1 (BST1) is a GPI-anchored molecule that promotes pre-B cell growth. Also known as ADP-ribosyl cyclase 2 and DAMP-1, it is expressed by the BST1 gene in humans. BST1 expression increases in bone marrow stromal cells from rheumatoid arthritis patients and may contribute to B cell abnormalities observed in the disease.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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